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Genetic modification by transgenesis and gene targeting has become a well-established method to generate mouse models for the study of gene functions. Functional annotation of these models is one of the biggest challenges in the current biomedical sciences. Accurate phenotyping of these models in the uniform genetic background is essential for this purpose. The C57BL/6N strain is one of the major C57BL/6 substrains together with C57BL/6J. In recent years, efforts have been made worldwide to generate knockout mouse resources of the C57BL/6N background. The germ cell competent C57BL/6N ES cell lines are available from the Cell Engineering Division of RIKEN BRC.
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MSM (RBRC00209) is a wild-derived inbred strain originated from Japanese wild mice, Mus musculus molossinus. The MSM mice have several unique phenotypes, such as small body size (<15 grams), low incidence of tumor development, and distinct behaviors, making them promising resources for higher brain functions and lifestyle-related diseases by expanding genome diversity. The MSM is especially invaluable for uncovering novel functions of the genome by the availability of the MSM genomic BAC libraries, whole genome sequence information, microsatellite marker data, consomic strains with C57BL/6 background, and germ cell competent ES cells. Thus, the MSM expands the range of the genetic variations of laboratory mouse strains.
Genome sequence of B6J: Build 37.1
Stats on BAC clones
BAC clones are available from RIKEN BioResource Center
by NBRP Mouse Genome Information Upgrading Program (RIKEN BRC, RIKEN Genomic Sciences Center,
and Comparative Genomics Laboratory, National Institute of Genetics) in Japan.